BLAST+ executables

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Do you have difficulties running high volume BLAST searches? Do you have proprietary sequence data to search and cannot use the NCBI BLAST web site? Do you have access to your own server? Do you have your own research pipeline? Have security or IP concerns about sending searches outside of your organization? If you answered yes to any of these questions, read on!

The NCBI provides a suite of command-line tools to run BLAST called BLAST+. Zodiac clock 3d screensaver. This allows users to perform BLAST searches on their own server without size, volume and database restrictions. BLAST+ can be used with a command line so it can be integrated directly into your workflow.

What are the next steps?

Download and install BLAST+. Installers and source code are available from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/. Download the databases you need,(see database section below), or create your own. Start searching.

For more details, please see the BLAST+ user manual, the BLAST Help manual, the BLAST releases notes, and the article in BMC Bioinformatics (PubMed link). See our versioning policy.

The BLAST+ suite is the currently supported package. The older C toolkit executables are no longer supported. See our versioning policy.

We are always listening and welcome your feedback at BLAST Support Center.

Magic-BLAST

Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome. Read more about Magic-BLAST at https://ncbi.github.io/magicblast.

Installers and source code are available from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/magicblast/LATEST.

IgBLAST

IgBLAST facilitates the analysis of immunoglobulin and T cell receptor variable domain sequences. For details please see the documentation at https://ncbi.github.io/igblast/ and the article in Nucleic Acids Research (https://www.ncbi.nlm.nih.gov/pubmed/23671333).

Installers and source code are available from ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/LATEST.

SRPRISM

SRPRISM is a short read alignment tool that works with genomic sequences and handles alternative loci. For more information, see ftp://ftp.ncbi.nlm.nih.gov/pub/agarwala/srprism/README. A LINUX executable is available under ftp://ftp.ncbi.nlm.nih.gov/pub/agarwala/srprism

Databases

BLAST databases are updated daily and may be downloaded via FTP fromftp://ftp.ncbi.nlm.nih.gov/blast/db/.Database sets may be retrieved automatically with update_blastdb.pl, which is part of the BLAST+ suite.Please refer to the BLAST database documentation for more details.

The NCBI makes searchable collection of position-specific scoring matrices that can be used for sensitive protein and translated nucleotide searches. These are known as the Conserved Domain Database and can be searched with the RPSBLAST and RPSTBLASTN executables distributed with the BLAST+ package. The most comprehensive collection of CDD's is available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/little_endian/Cdd_NCBI_LE.tar.gz. Subsets of the comprehensive collection are available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/little_endian/. Documentation on these CDD collections is available at ftp://ftp.ncbi.nlm.nih.gov/pub/mmdb/cdd/README.

Trimble Access 2020 was released this week and is available for Windows 10 controllers, and now for the first time, on the Trimble TDC600 powered by Android. View the release notes here, or download the new version using Trimble Installation Manager.

Trimble TDC600 Powered by Android

For surveyors looking to use a smart-phone style mobile device to collect data in the field, the Trimble TDC600 running Trimble Access 2020 provides an optimal solution with its lightweight, rugged design. It is also ideal for surveyors looking for a lower-cost platform with the new Trimble Access software and workflows.

The TDC600 can connect to all supported GNSS receivers and most conventional instruments using Bluetooth. To perform a robotic survey you will need to connect TDC600 to your instrument using the Trimble TDL2.4 radio.

Snap-to Toolbar

The Snap-to toolbar provides a simple way to select locations on objects in the map by snapping onto a specific point, even if no point exists. For example, you can use the Snap-to toolbar to accurately select the end point of a line or polyline or the center of an arc from linework in a map file, such as an IFC or DXF file.

To display the toolbar, tap on the map toolbar and then select Snap-to toolbar. The following Snap-to tools are available: Snap to midpoint, Snap to end, Snap to intersection, Snap to PI of arc, Snap to center, and Snap to nearest.

Select the required tool from the Snap-to toolbar and then select the line, arc, or polyline in the map to 'snap' to the appropriate location on the selected item. If a point does not already exist at the selected location, Trimble Access calculates a point. You can use calculated points like any other point, for example to stake out or to perform other Cogo functions. To be able to reuse a calculated point in the future, tap and hold in the map and select Create point to create a point from the calculated point and store it to the job.

The Snap-to toolbar is only available when you are using General Survey.

Limit Box

The Limit box enables you to exclude data in the map to view more clearly the area you are interested in. The Limit box is especially useful when viewing IFC files or point clouds, where you can exclude parts of the model or point cloud so that you can view inside the model or point cloud.

Trimble Access Feature Code Library

To display the Limit box tools, tap on the map toolbar and then select Limit box. The Limit box toolbar appears to the right of the map. You can set the size of the Limit box using the vertical, side, or front/back sliders, or by entering limit values.

To align the limit box sliders with the orientation of the scan data or IFC model, set the Reference azimuth for the job in the Cogo settings screen.

The following images show viewing an IFC file without the Limit box (left) and with the Limit box enabled (right):

Measuring points using independent subsets of tracked satellites

Trimble Feature Code Library Download

In RTK surveys, you can now measure points with independent subsets of the currently tracked GNSS satellite constellation. This allows for consecutive measurements to be independent of each other, without having to revisit the point after a significant period of time to ensure the constellation has changed. The SV subset function divides all tracked SVs into two subsets with an even spread throughout the sky, and can be used to make an occupation independent without having to return at another time. The SV subset function is available from the Satellites screen or the RTK Initialization screen.

Trimble Access 2020 Video Tutorials Playlist

Supported controllers

Windows 10 devices:

Trimble TSC7 controller
Trimble T7 or T10 tablet
Supported third-party tablets

Trimble Feature Code Library Download- Download Last Version 2017

Android devices:

Trimble TDC600

Installation Information

To install Trimble Access 2020.00 onto a supported controller you must have a Trimble Access software maintenance agreement valid up to 1 May 2020. To install the software to your controller, use the appropriate Trimble Installation Manager for your controller operating system.

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